Preparation of cloning vector and insert for ligation

. Enzyme specifically digests the agarose polysaccharide core which allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose.

Termosenzitivní alkalická fosfatáza katalyzuje odstraňování 5'- a 3'-fosfátových skupin z DNA, RNA, nukleotidů a proteinů.

Thermosensitive Alkaline Phosphatase catalyzes the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins.

Silica membrane-based DNA fragment purification from standard or low-melting point agarose gels run in either TAE or TBE buffer. Provided in a convenient spin-column format.

Silica membrane-based DNA fragment purification from standard or low-melting point agarose gels run in either TAE or TBE buffer. Provided in a convenient spin-column format.

GeneJET PCR Purification Kit is designed for rapid and efficient purification of DNA from PCR and other enzymatic reaction mixtures.

GeneJET PCR Purification Kit is designed for rapid and efficient purification of DNA from PCR and other enzymatic reaction mixtures.

The Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 µg of high copy plasmid DNA per isolation procedure.

The GeneJET Plasmid Miniprep Kit is a simple, rapid, and cost-effective system for the isolation of high quality plasmid DNA from recombinant E. coli cultures.

. FastDigest buffer for 100% activity Low concentration available O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 75°C in 10 min Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.