GeneJET™ Gel Extraction Kit

Detailní popis

Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

The kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column. The kit can be used to purify DNA fragments from 25 bp to 20 kb in size with recovery rates up to 95%. Each GeneJET purification column has a binding capacity of up to 25 µg of DNA and can process up to 1 g of agarose gel. The entire procedure takes just 15 minutes, and the isolated DNA is ready to use in all common downstream applications, including ligation, restriction digestion, PCR, sequencing, and labeling.

Highlights

• Highly efficient: up to 95% recoveries in the range of 100 bp to 10 kb
• Fast: procedure takes only 15 minutes
• Convenient: spin columns are capped and assembled with collection tubes

Applications

• Fast and efficient extraction of high purity DNA fragments ideal for use in all conventional molecular biology procedures including:
  • FastDigest or conventional restriction digestion
  • Automated fluorescent or radioactive sequencing
  • PCR
  • In situ hybridization
  • Labeling
  • Cloning

Includes

• Binding Buffer
• Wash Buffer (concentrated)
• Elution Buffer (10 mM Tris-HCl, pH 8.5)
• GeneJET Purification Columns (preassembled with collection tubes)
• Detailed Protocol