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5'...G C S G↓C...3'
3'...C↑G S C G...5'
Available as a FastDigest enzyme for rapid DNA digestion
The TauI restriction enzyme recognizes GCSG^C sites and cuts best at 55°C in B buffer
The GeneJET Gel Extraction and DNA Cleanup Micro Kit is developed as 3 in 1 kit designed for rapid and efficient purification of DNA from PCR, enzymatic reaction mixtures, and DNA extraction from standard or low-melting point agarose gels run in either Tris acetate (TAE) or Tris borate (TBE) buffer.
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Formula: C14H13BrClNO7 × C6N13N, Molecular Weight: 521.8
X-Gluc (5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid, cyclohexylammonium salt) is a substrate for β-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.
X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate.
Endonuclease V, T. maritima (Endo V) is a 3'-endonuclease involved in DNA repair, which initiates removal of deaminated bases from damaged DNA, including uracil, hypoxanthine, and xanthine.