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GeneJET™ Gel Extraction and DNA Cleanup Micro Kit

Kód produktu: K0832 Kód výrobce: K0832 Kód dodavatele: {075854A0-9D27-47AF-9DCE-69ADE0170705} Výrobce: Life Technologies Czech Republic s.r.o.
14 895,10 Kč
12 310,00 Kč bez DPH
do týdne
250 prep
The GeneJET Gel Extraction and DNA Cleanup Micro Kit is developed as 3 in 1 kit designed for rapid and efficient purification of DNA from PCR, enzymatic reaction mixtures, and DNA extraction from standard or low-melting point agarose gels run in either Tris acetate (TAE) or Tris borate (TBE) buffer.


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Detailní popis

The kit combines the convenience of spin column technology with the selective binding properties of a silica membrane, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

The GeneJET Gel Extraction and DNA Cleanup Micro Kit effectively removes primers, primer dimers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 100 bp to 20 kb. The recovery rates are 90 to 100% for 100 bp to 3 kb DNA fragment size range. Each GeneJET DNA Purification Column has a total binding capacity of up to 10 µg of DNA, and the entire procedure takes approximately 3.5 minutes for DNA cleanup


  • Advanced column design – easy to open, load and handle
  • Yields concentrated DNA - Elute in 6 to 10 µL volume
  • High recovery - 90 to 100% recovery in the 100 to 3000 bp DNA fragment range
  • Fast – 4 minute reaction mixture clean-up and 15 minute agarose gel purification protocols available
  • Efficient removal of reaction contaminants - including enzymes, proteins, primers dimers, dNTPs, and salts
  • Flexible – does not require precise gel slice quantification weighting


The purified DNA can be used in common downstream applications:

  • Sequencing
  • Restriction digestion
  • PCR
  • qPCR
  • Labeling
  • Ligation
  • Cloning
  • In vitro transcription
  • Blotting
  • In situ hybridization


  • Binding Buffer
  • Extraction Buffer
  • Prewash Buffer (conc.)
  • Wash Buffer (conc.)
  • Elution Buffer
Hazardous No
Quality Control The Thermo Scientific GeneJET Gel Extraction and DNA Cleanup Micro Kit is qualified by isolating 3 kb DNA fragment from 200 µL reaction mixture and extracting 3 kb DNA from 1% agarose gel according the protocols outlined in the manual. The quality of the purified and extracted DNA is evaluated spectrophotometrically and by agarose gel electrophoresis.
Storage Condition Ambient

Obsah balení

250 preps

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