CpG Methyltransferase (M.SssI)
Kód produktu: EM0821
Kód výrobce: EM0821
Kód dodavatele: {E80F33E0-E709-4FDF-8298-B98884982462}
Výrobce:
Life Technologies Czech Republic s.r.o.
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CpG Methyltransferase (M.SssI) is one of the basic tools used in epigenetic studies.
Detailní popis
The enzyme methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5’-CpG-3’ context.
Thermo Scientific M.SssI is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes modification of all CpGs in 15 minutes at 37°C. In addition, the enzyme has been specifically validated for use with genomic DNA – the primary substrate in epigenetic studies.
M.SssI is supplied with an optimized 10X reaction buffer and 50X S-adenosylmethionine (SAM) as a cofactor.
Highlights
- High efficiency – complete in vitro methylation of all CpGs in non-methylated and hemi-methylated DNA
- Fast – reaction is completed in 15 minutes at 37°C
- Stable – at -20°C for 2 years
Applications
- Epigenetics studies
- in vitro methylation of DNA for methylation analysis
- Inhibition of endonucleases with overlapping CpG sequence recognition.
- [3H]-labeling of DNA
| Formulation | One µL of M.SssI protects 1 µg of genomic DNA from digestion with HpaII in 15 min at 37°C in 50 µL of recommended reaction buffer. |
| Hazardous | No |
| Quality Control | Prolonged incubation No detectable change in DNA fragment band pattern observed following agarose gel electrophoresis of SmaI linearized pUC19 DNA incubated for 16 hours with 1 µL of M.SssI. Labeled Oligonucleotide (LO) Assay No detectable degradation of single-stranded or double-stranded labeled oligonucleotides observed after incubation with 1 µL of M.SssI for 4 hours at 37°C, analysis by polyacrylamide gel electrophoresis and phosphoimaging. |
| Storage Buffer | CpG Methyltransferase (M.SssI) is supplied in 10 mM potassium phosphate (pH 7.0 at 25°C), 400 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA, and 50% glycerol. |
| Storage Condition | -20 C |
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