X-Gluc

Kód produktu: R0851 Kód výrobce: R0851 Kód dodavatele: {59F8F222-97B0-4CA8-A9AB-649C18F6E329} Výrobce: UAB Fermentas
3 127,85 Kč
2 585,00 Kč bez DPH
do týdne
0,1 g

Formula: C14H13BrClNO7 × C6N13N, Molecular Weight: 521.8

X-Gluc (5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid, cyclohexylammonium salt) is a substrate for β-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.
Zobraz detailní popis

Detailní popis

Thermo Scientific X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).

Applications

  • Detection of GUS expression in plant cells and tissues (see Reference 1)
  • Detection of infections caused by E. coli
  • Detection of bacterial contamination in food and water samples.
Glucuronidase cleaves X-Gluc

Glucuronidase cleaves X-Gluc

Note

Preparation of a 0.1 M stock solution in dimethylformamide or dimethylsulfoxide is recommended.

CategoryName
Formula C14H13BrClNO7 × C6N13N
Hazardous No
Hazardous: No
Molecular Weight 521.8
Quality Control Purity of ≥ 97% confirmed by HPLC
Shelf Life:
Shipping Condition:
Storage Condition -20 C
Storage Condition: -20 C
Storage Note Store at -20°C in the dark.

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