Endonuclease V, T.maritima

Kód produktu: EN0141 Kód výrobce: EN0141 Kód dodavatele: {5E003C8E-AA76-43FD-8767-00091273E42F} Výrobce: UAB Fermentas
2 595,45 Kč
2 145,00 Kč bez DPH
do týdne
250 Units
Endonuclease V, T. maritima (Endo V) is a  3'-endonuclease involved in DNA repair, which initiates removal of deaminated bases from damaged DNA, including uracil, hypoxanthine, and xanthine.
Zobraz detailní popis

Detailní popis

Thermo Scientific Endonuclease V, T. maritima (Endo V) is a  3'-endonuclease involved in DNA repair, which initiates removal of deaminated bases from damaged DNA, including uracil, hypoxanthine, and xanthine (see Figure 1).

Endonuclease V is also active toward abasic sites and urea sites, base pair mismatches, flap and pseudo Y structures, and small insertions/deletions in DNA molecules. The cleavage site generated by Endonuclease V is at the second phosphodiester bond 3' to a lesion. 

Highlights

  • Optimal activity at temperatures of 65 to 70°C

Applications

  • High-throughput methods for mutation research (see​ References 3,4)
  • Studies in mutagenesis and DNA repair
  • Mismatch cleavage
  • Genotyping

Note

Use of this enzyme in certain applications may be covered by patents and may require a license. 

When the enzyme is in excess, the primary nicked products experience a  second nicking event on the complementary strand, leading to a  double-stranded break. At low concentrations, however, Endonuclease V  first nicks a DNA strand at the lesions located closer to the 5'-end of DNA molecule. Single-stranded DNA is cleaved with much lower efficiency. Mg2+ or Mn2+ ions are required for enzyme activity (see References 1-3).

10X Reaction Buffer 500 mM Tris-acetate (pH 7.5), 500 mM KCl, 10 mM EDTA, 0.5% (v/v) Triton X-100.
CategoryName
Concentration 5 U/µL
Definition of Activity Unit
  • One unit of the enzyme converts 1 µg of supercoiled depurinized plasmid DNA into other topological states in 30 min at 65°C.
  • Enzyme activity is assayed in the following mixture: 25 mM HEPES-NaOH (pH 7.4), 5 mM MgCl2, 5 mM DTT, 2% (v/v) glycerol, 2 µg of partially depurinated pUC19 DNA.
Hazardous No
Hazardous: No
Inactivation Inactivated by heating in boiling water bath for 10 min, preferably in the presence of EDTA.
Inhibition Inhibitors: no specific inhibitors have been described.
Molecular Weight 25 kDa monomer.
Quality Control The absence of other endo-, exodeoxyribonucleases, and ribonucleases confirmed by appropriate quality tests.
Shelf Life:
Shipping Condition:
Shipping Information
Source E.coli with a cloned nfi gene of Thermotoga maritima 
SpecificationName
SpecificationValue
Storage Buffer The enzyme is supplied in:
20 mM HEPES-NaOH (pH 7.4), 5 mM DTT, 50 mM NaCl, 0.1% (v/v) Triton X-100 and 50% (v/v) glycerol.
Storage Condition -20 C
Storage Condition: -20 C

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