Thermo Scientific Endonuclease V, T. maritima (Endo V) is a 3'-endonuclease involved in DNA repair, which initiates removal of deaminated bases from damaged DNA, including uracil, hypoxanthine, and xanthine (see Figure 1).
Endonuclease V is also active toward abasic sites and urea sites, base pair mismatches, flap and pseudo Y structures, and small insertions/deletions in DNA molecules. The cleavage site generated by Endonuclease V is at the second phosphodiester bond 3' to a lesion.
- Optimal activity at temperatures of 65 to 70°C
- High-throughput methods for mutation research (see References 3,4)
- Studies in mutagenesis and DNA repair
- Mismatch cleavage
Use of this enzyme in certain applications may be covered by patents and may require a license.
When the enzyme is in excess, the primary nicked products experience a second nicking event on the complementary strand, leading to a double-stranded break. At low concentrations, however, Endonuclease V first nicks a DNA strand at the lesions located closer to the 5'-end of DNA molecule. Single-stranded DNA is cleaved with much lower efficiency. Mg2+ or Mn2+ ions are required for enzyme activity (see References 1-3).
|10X Reaction Buffer||500 mM Tris-acetate (pH 7.5), 500 mM KCl, 10 mM EDTA, 0.5% (v/v) Triton X-100.|
|Definition of Activity Unit||
|Inactivation||Inactivated by heating in boiling water bath for 10 min, preferably in the presence of EDTA.|
|Inhibition||Inhibitors: no specific inhibitors have been described.|
|Molecular Weight||25 kDa monomer.|
|Quality Control||The absence of other endo-, exodeoxyribonucleases, and ribonucleases confirmed by appropriate quality tests.|
|Source||E.coli with a cloned nfi gene of Thermotoga maritima|
|Storage Buffer||The enzyme is supplied in:
20 mM HEPES-NaOH (pH 7.4), 5 mM DTT, 50 mM NaCl, 0.1% (v/v) Triton X-100 and 50% (v/v) glycerol.
|Storage Condition||-20 C|
|Storage Condition:||-20 C|
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