Preparation of cloning vector and insert for ligation

The Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 µg of high copy plasmid DNA per isolation procedure.

Termosenzitivní alkalická fosfatáza katalyzuje odstraňování 5'- a 3'-fosfátových skupin z DNA, RNA, nukleotidů a proteinů.

Thermosensitive Alkaline Phosphatase catalyzes the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins.

The GeneJET Plasmid Miniprep Kit is a simple, rapid, and cost-effective system for the isolation of high quality plasmid DNA from recombinant E. coli cultures.

Silica membrane-based DNA fragment purification from standard or low-melting point agarose gels run in either TAE or TBE buffer. Provided in a convenient spin-column format.

GeneJET PCR Purification Kit is designed for rapid and efficient purification of DNA from PCR and other enzymatic reaction mixtures.

Silica membrane-based DNA fragment purification from standard or low-melting point agarose gels run in either TAE or TBE buffer. Provided in a convenient spin-column format.

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA.

GeneJET PCR Purification Kit is designed for rapid and efficient purification of DNA from PCR and other enzymatic reaction mixtures.

. B buffer for 100% activity FastDigest buffer for 100% activity G buffer for 100% activity O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 75°C in 10 min T4 DNA Polymerase T4 DNA Polymerase is template-dependent and catalyzes the 5'-3' synthesis from primed single-stranded DNA.