Preparation of cloning vector and insert for ligation

Thermosensitive Alkaline Phosphatase catalyzes the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins.

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA

S1 Nuclease is an endonuclease which degrades single-stranded DNA and RNA.

. B buffer for 100% activity FastDigest buffer for 100% activity G buffer for 100% activity O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 75°C in 10 min T4 DNA Polymerase T4 DNA Polymerase is template-dependent and catalyzes the 5'-3' synthesis from primed single-stranded DNA.

. FastDigest buffer for 100% activity Low concentration available O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 75°C in 10 min Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

The GeneJET Plasmid Miniprep Kit is a simple, rapid, and cost-effective system for the isolation of high quality plasmid DNA from recombinant E. coli cultures.

GeneJET PCR Purification Kit is designed for rapid and efficient purification of DNA from PCR and other enzymatic reaction mixtures.

Silica membrane-based DNA fragment purification from standard or low-melting point agarose gels run in either TAE or TBE buffer. Provided in a convenient spin-column format.

. Enzyme specifically digests the agarose polysaccharide core which allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose.

Silica bead-based DNA extraction from agarose gels and reaction mixtures. Flexible to scale up or down both reaction and elution volumes.