S1 Nuclease
Kód produktu: EN0321
Kód výrobce: EN0321
Kód dodavatele: {2C4E1AF7-C5F3-4914-909B-B2553CB999A5}
Výrobce:
Life Technologies Czech Republic s.r.o.
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S1 Nuclease is an endonuclease which degrades single-stranded DNA and RNA.
Detailní popis
Thermo Scientific S1 Nuclease degrades single-stranded nucleic acids, releasing 5'-phosphoryl mono- or oligonucleotides (see Figure 1 in Supporting Data). It is five times more active on DNA than on RNA (see Reference 1). S1 Nuclease also cleaves dsDNA at the single-stranded region caused by a nick, gap, mismatch or loop. S1 Nuclease exhibits 3'-phosphomonoesterase activity.
The enzyme is a glycoprotein with carbohydrate content of 18%.
Applications
- Removal of single-stranded overhangs of DNA fragments (see Reference 2)
- S1 transcript mapping (see References 3, 4)
- Cleavage of hairpin loops
- Creation of unidirectional deletions in DNA fragments in conjunction with Exonuclease III (see Reference 5)
Note
S1 Nuclease can introduce breaks into double-stranded DNA, RNA and DNA/RNA hybrids at high enzyme and low salt concentrations (see Reference 6).
| 5X Reaction Buffer | 200 mM sodium acetate (pH 4.5 at 25°C), 1.5 M NaCl, and 10 mM ZnSO4. |
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| Concentration | 100 U/µL |
| Definition of Activity Unit | One unit of the enzyme produces 1 µg of acid soluble deoxyribonucleotides in 1 min at 37°C. |
| Hazardous | No |
| Hazardous: | No |
| Inactivation | Inactivated by heating at 70°C for 10 min in the presence of EDTA |
| Inhibition | Inhibitors: metal chelators, PPi, Pi, 5'-ribonucleotides, and deoxyribonucleotides |
| Molecular Weight | 29 kDa monomer |
| Quality Control |
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| Shipping Information | |
| Source | Aspergillus oryzae |
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| Storage Buffer | The enzyme is supplied in 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 0.1 mM ZnCl2, and 50% (v/v) glycerol. |
| Storage Condition | -20 C |
| Storage Condition: | -20 C |
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