T4 DNA Polymerase

Kód produktu: EP0061 Kód výrobce: EP0061 Kód dodavatele: {2BF78BA7-9B3C-4A33-A42C-8FFBC2B89F81} Výrobce: UAB Fermentas
1 304,38 Kč
1 078,00 Kč bez DPH
do týdne
100 Units


B buffer for 100% activity FastDigest buffer for 100% activity G buffer for 100% activity O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 75°C in 10 min

T4 DNA Polymerase

T4 DNA Polymerase is template-dependent and catalyzes the 5'-3' synthesis from primed single-stranded DNA.

Zobraz detailní popis

Detailní popis

Thermo ScientificT4 DNA Polymerase, a template-dependent DNA polymerase, catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity.


  • Stronger 3'-5' exonuclease activity on single-stranded than on double-stranded DNA and greater (more than 200 times) than DNA polymerase I, E. coli,and Klenow fragment (see Reference1)
  • Active in Thermo Scientific restriction enzyme, PCR, RT and T4 DNA Ligase buffers


  • Blunting of DNA ends: fill-in of 5'-overhangs or/and removal of 3'-overhangs(see​ References1, 2)
  • Blunting of PCR products with 3'-dA overhangs
  • Synthesis of labeled DNA probes by the replacement reaction(see​ Reference3)
  • Oligonucleotide-directed site-specific mutagenesis(see​ Reference4)
  • Ligation-independent cloning of PCR products(see​ References5, 6)
    5× Reaction Buffer 335mM Tris-HCl (pH8.8 at 25°C), 33mM MgCl2, 5mM DTT, 84mM (NH4)2SO4
    Concentration 5U/µL
    Definition of Activity Unit
    • One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30min at 37°C.
    • Enzyme activity is assayed in the following mixture: 67mM Tris-HCl (pH 8.8), 6.7mM MgCl2, 1mM DTT, 16.7mM (NH4)2SO4, 0.2mg/mL BSA, 0.033mM of each dNTP, 0.4 MBq/mL [3H]-dTTP, and 0.2mM heat-denatured and nuclease-digested calf thymus DNA.
    Hazardous No
    Inactivation Inactivated by heating at 75°C for 10min.
    Inhibition Inhibitors: metal chelators, nucleotide analogs 2(p-n-butylanilino)-dATP, N2-(p-n-butylphenyl)-dGTP), SH-blocking compounds (see Reference 7)
    Molecular Weight 104kDa monomer
    Quality Control The absence of endodeoxyribonucleases confirmed by appropriate quality tests.
    Source E.coli cells with a cloned gene 43 of bacteriophage T4
    Storage Buffer The enzyme is supplied in 20mM potassium phosphate (pH7.5), 200mM KCl, 2mM DTT, and 50%(v/v) glycerol.
    Storage Condition -20 C

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