Reagencie - Life Technologies Czech Republic s.r.o.

10x Buffer O 10X Buffer O ensures the optimum reaction conditions for restriction enzymes. Premixed with BSA for enhanced stability.

dNTP/dUTP Mix is an aqueous solution containing dATP, dCTP, dGTP, each at a final concentration of 2 mM and dUTP of 4 mM

10X Taq DNA Polymerase buffers with different compositions

5'...G A A G A (N)8↓...3' 3'...C T T C T (N)7↑...5'

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5'...C C A N N N N↓N T G G...3' 3'...G G T N↑N N N N A C C...5'

5'...G↓C G C...3' 3'...C G C↑G...5'

5'-d(GTAAAACGACGGCCAGT)-3' M13 pUC sequencing primers are single-stranded oligonucleotides with 5'-hydroxyl and 3'-hydroxyl ends.

5'...G m6A↓  T C...3' 3'...C   T↑m6A G...5'

5'...C A G↓C T G...3' 3'...G T C↑G A C...5' 

A conventional marker composed of 11 sharp bands for sizing and approximate quantification of small double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels.

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle). The kit also includes Thermo Scientific DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.