phiX174 DNA/BsuRI (HaeIII) Marker

Detailní popis

Thermo ScientificphiX174 DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme, then purified and dissolved in storage buffer. The DNA fragments contain blunt or sticky ends, depending on the restriction enzyme used for the markers preparation.

Thermo Scientific phiX174 DNA/BsuRI (HaeIII) Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction, see protocols in Resources

Highlights

• Sizing and approximate quantification of small DNA fragments
• Sharp bands 
• Supplied with loading dye for sample DNA 

Note

• Bands indicated ** form an anomalous pattern on polyacrylamide gels.
• The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of the respective DNA sequences.