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Kód produktu: ER0631 Kód výrobce: ER0631 Kód dodavatele: {D5F9FDE3-9770-4D93-A564-BEEA5DF0C46B} Výrobce: Life Technologies Czech Republic s.r.o.
1 323,74 Kč
1 094,00 Kč bez DPH
2500 Unit

5'...C A GC T G...3'
3'...G T CG A C...5'

Zobraz detailní popis

Detailní popis

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.


  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities


  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP


For methylation sensitivity refer to product specifications.

Conditions for 100% Activity
  • 1X Buffer G: 10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 50 mM NaCl, and 0.1 mg/mL BSA.
  • Incubate at 37°C
Digestion of Agarose-embedded DNA Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
Double Digestion Perform double digestion using DoubleDigest.
Hazardous No
Isoschizomers Search for commercial isoschizomers using REsearch.
Note Greater than 15-fold overdigestion with PvuII may result in star activity.
Storage Buffer PvuII is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/mL BSA, and 50% (v/v) glycerol.
Storage Condition -20 C

Reaction conditions

Recommended buffer for 100% activity Optimal temperature Enzyme activity in Thermo Scientific buffers, % Tango buffer for double digestion
B (blue)
G (green)
O (orange)
R (red)
1X / 2X
G (Green) 37°C 50-100† 100 20-50 50-100 20-50† 20-50† 1X† or 2X†

† star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Methylation Effects

  • Dam: never overlaps – no effect.
  • Dcm: never overlaps – no effect.
  • CpG: never overlaps – no effect.
  • EcoKI: never overlaps – no effect.
  • EcoBI: may overlap – effect not determined.

Cleavage efficiency close to the termini of PCR fragments

bp from the recognition site to fragment end
1 2 3 4 5

Number of recognition sites in DNA molecules

Lambda ΦX174 M13mp18/19 pBR322 puc18/19 pUC57
15 0 3 1 2 2
pTZ19R/U pTZ57R pBluescriptIIKS(±) pBluescriptIISK(±) pACYC177 pACYC184
2 2 2 2 0 2

New sites generated by ligation

Newly generated recognition sites resulting from ligation of blunt DNA ends

Recognition sequence Second restriction enzyme Restriction enzymes cleaving the newly generated recognition sequence
  • AjiI (BmgBI)* (CAC^GTC)
  • AjiI (BmgBI)* (GAC^GTG)
  • Eco105I (SnaBI)/FastDigest SnaBI (Eco105I) (TAC^GTA)
  • Eco72I (PmlI)/FastDigest PmlI (Eco72I) (CAC^GTG)
  • Ppu21I (BsaAI)/FastDigest BsaAI (Ppu21I)* (YAC^GTA)
  • Ppu21I (BsaAI)/FastDigest BsaAI (Ppu21I)* (YAC^GTG)
  • ZraI (GAC^GTC)
  • SetI
  • AluI/FastDigest AluI (AG^CT)
  • CviJI* (RG^CT)
  • Ecl136II (EcoICRI)/FastDigest Ecl136II (GAG^CTC)
  • MbiI (BsrBI)/FastDigest BsrBI (MbiI)* (CCG^CTC)
  • AluI/FastDigest AluI
  • CviJI
  • SetI
  • Bst1107I (BstZ17I)/FastDigest BstZ17I (Bst1107I) (GTA^TAC)
  • Csp6I (CviQI)/FastDigest Csp6I
  • RsaI/FastDigest RsaI
  • BsuRI (HaeIII)/FastDigest HaeIII (BsuRI) (GG^CC)
  • CviJI* (RG^CC)
  • Eco147I (StuI)/FastDigest StuI (Eco147I) (AGG^CCT)
  • Eco47III (AfeI)/FastDigest AfeI (Eco47III) (AGC^GCT)
  • MlsI (MscI)/FastDigest MscI (MlsI) (TGG^CCA)
  • CviJI
  • Eco32I (EcoRV)/FastDigest EcoRV (Eco32I) (GAT^ATC)
  • Bsp143I (Sau3AI)/FastDigest Sau3AI (Bsp143I)
  • DpnI/FastDigest DpnI
  • MboI/FastDigest MboI
  • EheI (SfoI)/FastDigest EheI (GGC^GCC)
  • BsuRI (HaeIII)/FastDigest HaeIII (BsuRI)
  • CviJI
  • FaiI* (YA^TG)
  • TscAI (TspRI)/FastDigest TspRI (TscAI)
  • MbiI (BsrBI)/FastDigest BsrBI (MbiI)* (GAG^CGG)
  • MspA1I* (CMG^CGG)
  • MspA1I
  • MspA1I* (CMG^CTG)
  • AluI/FastDigest AluI
  • CviJI
  • MspA1I
  • PvuII/FastDigest PvuII
  • SetI

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