M13/pUC Sequencing Primer (-20), 17-mer
Kód produktu: SO100
Kód výrobce: SO100
Kód dodavatele: {8A13C93B-54AB-4DEB-9DE3-69B94FB6A884}
Výrobce:
Life Technologies Czech Republic s.r.o.
5'-d(GTAAAACGACGGCCAGT)-3'
M13 pUC sequencing primers are single-stranded oligonucleotides with 5'-hydroxyl and 3'-hydroxyl ends.
Detailní popis
The Thermo Scientific M13/pUC sequencing primers anneal to the region in the 5'-terminus of the lacZ gene. All primers are supplied as 10 µM aqueous solutions.
Applications
- Sequencing of DNA fragments inserted into the MCS within the lacZ gene of various cloning vectors, such as pUC19, pTZ19R, pTZ57R, M13mp18, and pBluescript II
- Colony screening by PCR
Hazardous | No |
Quality Control |
Functionally tested by dideoxy sequencing of pUC19 DNA. |
Storage Condition | -20 C |
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