Properties of mesophilic DNA poymerases

. B buffer for 100% activity FastDigest buffer for 100% activity G buffer for 100% activity O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 75°C in 10 min T4 DNA Polymerase T4 DNA Polymerase is template-dependent and catalyzes the 5'-3' synthesis from primed single-stranded DNA.

. FastDigest buffer for 100% activity    LO certified O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 75°C in 10 min Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.

. FastDigest buffer for 100% activity Low concentration available O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 75°C in 10 min Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

. Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.

Recombinant enzyme Thermal inactivation at 65°C in 10 min phi29 DNA Polymerase is a highly processive polymerase with strong strand displacement activity that allows for highly efficient isothermal DNA amplification.

. B buffer for 100% activity FastDigest buffer for 100% activity G buffer for 100% activity O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 75°C in 10 min T4 DNA Polymerase T4 DNA Polymerase is template-dependent and catalyzes the 5'-3' synthesis from primed single-stranded DNA.

. Recombinant enzyme Thermal inactivation at 65°C in 10 min phi29 DNA Polymerase is a highly processive polymerase with strong strand displacement activity that allows for highly efficient isothermal DNA amplification.

. B buffer for 100% activity FastDigest buffer for 100% activity G buffer for 100% activity O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 75°C in 10 min T7 DNA Polymerase, a template dependent DNA polymerase, catalyzes DNA synthesis in the 5'=>3' direction.

Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar in DNA and prevents carry-over of DNA in PCR reactions.

. Recombinant enzyme Thermal inactivation at 65°C in 10 min phi29 DNA Polymerase is a highly processive polymerase with strong strand displacement activity that allows for highly efficient isothermal DNA amplification.