DNA Polymerase I

Kód produktu: EP0041 Kód výrobce: EP0041 Kód dodavatele: {3A72DBDB-8FB5-44D3-AD8C-716BEB7C8F59} Výrobce: UAB Fermentas
1 766,60 Kč
1 460,00 Kč bez DPH
do týdne
500 Units

Detailní popis

Thermo Scientific DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonuclease (proofreading) activity, 5'→3' exonuclease activity, and ribonuclease H activity.

Highlights

  • Incorporates modified nucleotides (e.g. biotin-, digoxigenin-, aminoallyl-, fluorescently-labeled nucleotides)
  • Active in multiple buffers, including restriction enzyme, PCR, and RT buffers

Applications

  • DNA labeling by nick-translation in conjunction with DNase (see References 1-3)
  • Second-strand synthesis of cDNA in conjunction with RNase H (see Reference 4)
    10X Reaction Buffer 500 mM Tris-HCl (pH 7.5 at 25°C), 100 mM MgCl2, 10 mM DTT.
    CategoryName
    Definition of Activity Unit
    • One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 37°C, using poly(dA-dT)·poly(dA-dT) as a template·primer.
    • Enzyme activity is assayed in the following mixture: 67 mM potassium phosphate (pH 7.4), 6.7 mM MgCl2, 1 mM 2-mercaptoethanol, 0.033 mM dATP, 0.033 mM dTTP, 0.4 MBq/mL [3H]-dTTP, and 62.5 µg/mL poly(dA-dT)·poly(dA-dT).
    Hazardous No
    Hazardous: No
    Inactivation Inactivated by heating at 75°C for 10 min or by addition of EDTA.
    Inhibition Inhibitors: metal chelators, PPi, Pi (at high concentrations) (see Reference 5).
    Molecular Weight 103 kDa monomer
    Quality Control The absence of endodeoxyribonucleases confirmed by appropriate quality test.
    Shelf Life:
    Shipping Condition:
    Shipping Information
    Source E.coli cells with a cloned polA gene.
    SpecificationName
    SpecificationValue
    Storage Buffer The enzyme is supplied in 25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) glycerol.
    Storage Condition -20 C
    Storage Condition:

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