Reagencie - Life Technologies Czech Republic s.r.o.

An endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.

B buffer for 100% activity Blue/white certified FastDigest buffer for 100% activity G buffer for 100% activity High concentration available                                        LO certified O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 65°C in 10 min T4 DNA Ligase catalyzes the formation of phosphodiester bonds between 5‘-P and 3‘-OH of dsDNA fragments.

5'...G A C N↓N N G T C...3' 3'...C T G N N↑N C A G...5'

5'...G↓C G C G C...3' 3'...C G C G C↑G...5'

10X TBE Buffer (Tris-borate-EDTA) 10X TBE Buffer (Tris-borate-EDTA) is used in electrophoresis of nucleic acids in agarose and polyacrylamide gels.

10x Buffer BfuI Optimal buffer recommended for use with BfuI restriction enzyme. Premixed with BSA for enhanced stability.

dNTP mixes – premixed aqueous solutions of dATP, dTTP, dCTP, and dGTP.

2X RNA Loading Dye is recommended for preparation of RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels.

RNase A/T1 Mix combines the RNA degradation activity of both RNase A and RNase T1.

10x Buffer Eco52I Optimal buffer recommended for use with Eco52I restriction enzyme. Premixed with BSA for enhanced stability.

10x Buffer SduI, Ppu21I Optimal buffer recommended for use with SduI, Ppu21I restriction enzymes. Premixed with BSA for enhanced stability.

10x Buffer SdaI Optimal buffer recommended for use with SdaI restriction enzyme. Premixed with BSA for enhanced stability.