10X TBE Buffer (Tris-borate-EDTA)

Kód produktu: B52 Kód výrobce: B52 Kód dodavatele: {4E84C786-51B2-4FD5-A2AD-923F2EE2CAC8} Výrobce: UAB Fermentas
1 156,76 Kč
956,00 Kč bez DPH
do týdne
1 l

10X TBE Buffer (Tris-borate-EDTA)

Store at room temperature
10X TBE Buffer (Tris-borate-EDTA) is used in electrophoresis of nucleic acids in agarose and polyacrylamide gels.
Zobraz detailní popis

Detailní popis

Thermo Scientific 10X TBE Buffer (Tris-borate-EDTA) is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels, but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer (Tris Acetate-EDTA buffer, 10X powder, sc-296647) is recommended when looking at enzymatic applications for the DNA sample.

Applications

  • Electrophoresis of nucleic acids in agarose and polyacrylamide gels
  • Used both as a running buffer and as a gel preparation buffer
  • Filtered through a 0.22 µm membrane
  • Recommended for electrophoresis of RNA and DNA fragments smaller than 1500 bp

Note

Double-stranded linear nucleic acid molecules migrate about 10% slower in TBE buffer than in TAE buffer.

1X Composition 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH of 10X TBE: 8.3
Hazardous No
Quality Control The absence of endo-, exodeoxyribonucleases, and ribonucleases confirmed by appropriate quality tests.
Storage There are no time limitations for storage of electrophoresis buffers at room temperature. If the buffer is stored at lower temperatures, a  precipitate may form, which is easily dissolved by gentle heating.
Storage Condition Ambient
Usage Recommendations
  • Use fresh 1X TBE both for the gel and for the electrophoresis run.
  • To prepare 1X TBE buffer, add 100 mL of 10X TBE buffer to 900 mL of deionized water and mix well.

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