Generation of template DNA for in vitro transcription

dNTP mixes – premixed aqueous solutions of dATP, dTTP, dCTP, and dGTP.

. B buffer for 100% activity FastDigest buffer for 100% activity G buffer for 100% activity O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 75°C in 10 min T4 DNA Polymerase T4 DNA Polymerase is template-dependent and catalyzes the 5'-3' synthesis from primed single-stranded DNA.

The Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

The Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

Thermosensitive Alkaline Phosphatase catalyzes the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins.

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The Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 µg of high copy plasmid DNA per isolation procedure.

  CloneJET PCR Cloning Kit is a positive selection system for efficient cloning of PCR products generated with any thermostable DNA polymerase.

TA Cloning kit designed for cloning directly from a PCR reaction. It takes only one hour from the completion of PCR to cell plating.

The GeneJET Plasmid Miniprep Kit is a simple, rapid, and cost-effective system for the isolation of high quality plasmid DNA from recombinant E. coli cultures.

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between 5‘-P and 3‘-OH of dsDNA fragments.