Rapid DNA Ligation Kit

Detailní popis

The Thermo Scientific Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature (see Figure 1 in Supportig Data).

The kit contains T4 DNA ligase and a specially-formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation with the TransformAid Bacterial Transformation Kit or with other conventional transformation procedures.

Highlights

• Fast – ligation of either sticky- or blunt-end DNA in only 5 minutes
• Convenient – reaction mixture can be used directly for bacterial transformation

Applications

• Routine cloning experiments
• Blunt-end cloning
• Library construction
• TA cloning

Includes

• T4 DNA Ligase
• 5X Rapid Ligation Buffer
• Water, nuclease-free
• Detailed Protocol

Note

Prior to electroporation, it is necessary to inactivate T4 DNA ligase (see Reference 1) by chloroform extraction or spin column purification, e.g. with Thermo Scientific GeneJET PCR Purification Kit (K0701).