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XceI (NspI)

Kód produktu: ER1471 Kód výrobce: ER1471 Kód dodavatele: {7C8FD256-F3B6-4645-A02B-D90E803C12DB} Výrobce: Life Technologies Czech Republic s.r.o.
2 934,25 Kč
2 425,00 Kč bez DPH
do týdne
500 Units


5'...R C A T GY...3'
3'...YG T A C R...5'

Zobraz detailní popis
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Detailní popis

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.


  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities


  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP


For methylation sensitivity refer to product specifications.

Compatible Ends PaeI, Hin1II.
Conditions for 100% Activity
  • 1X Buffer Tango: 33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate, and 0.1 mg/mL BSA
  • Incubate at 37°C
Double Digestion Perform double digestion using DoubleDigest.
Hazardous No
Isoschizomers Search for commercial isoschizomers using REsearch.
Storage Buffer XceI is supplied in 10 mM Tris-HCl (pH 7.5 at 25°C), 300 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA, and 50% (v/v) glycerol.
Storage Condition -20 C

Reaction conditions

Recommended buffer for 100% activity Optimal temperature Enzyme activity in Thermo Scientific buffers, % Tango buffer for double digestion
B (blue)
G (green)
O (orange)
R (red)
1X/ 2X
Tango 37°C 50-100 0-20 0-20 0-20 100 0-20 1X

Methylation Effects

  • Dam: never overlaps - no effect.
  • Dcm: never overlaps - no effect.
  • CpG: may overlap - no effect.
  • EcoKI: never overlaps - no effect.
  • EcoBI: never overlaps - no effect.
Methylation type Sequence Cleavage effect
CpG 5'...RCATGm5C G...3'
3'...YGTAC Gm5C...5'
No effect
CpG 5'...m5C GCATGm5C G...3'
3'... Gm5CGTAC Gm5C...5'
Not determined

Cleavage efficiency close to the termini of PCR fragments

bp from the recognition site to fragment end
1 2 3 4 5
0 50-100

Number of recognition sites in DNA molecules

Lambda ΦX174 M13mp18/19 pBR322 puc18/19 pUC57
32 0 6 4 3 3
pTZ19R/U pTZ57R pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
2 2 1 1 1 2

New sites generated by ligation

Newly generated recognition sites resulting from ligation of protruding compatible DNA ends

Recognition Sequence Second Enzyme Enzymes recognizing newly generated recognition sequence
  • Hin1II (NlaIII)/FastDigest NlaIII (Hin1II) (CATG)
  • CviAII
  • FaiI
  • FatI
  • Hin1II (NlaIII)/FastDigest NlaIII (Hin1II)
  • PaeI (SphI)/FastDigest SphI (PaeI) (GCATG^C)
  • CviAII
  • FaiI
  • FatI
  • Hin1II (NlaIII)/FastDigest NlaIII (Hin1II)
  • XceI (NspI)/FastDigest NspI (XceI)
  • Hin1II (NlaIII)/FastDigest NlaIII (Hin1II) (CATG)
  • CviAII
  • FaiI
  • FatI
  • Hin1II (NlaIII)/FastDigest NlaIII (Hin1II)
  • PaeI (SphI)/FastDigest SphI (PaeI) (GCATG^C)
  • Cac8I
  • CviAII
  • FaiI
  • FatI
  • Hin1II (NlaIII)/FastDigest NlaIII (Hin1II)
  • PaeI (SphI)/FastDigest SphI (PaeI)
  • XceI (NspI)/FastDigest NspI (XceI)

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