X-Gluc
Kód produktu: R0852
Kód výrobce: R0852
Kód dodavatele: {13A7F5C0-FD7E-461D-88AE-481CCEBC6E25}
Výrobce:
Life Technologies Czech Republic s.r.o.
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Formula: C14H13BrClNO7 × C6N13N, Molecular Weight: 521.8
X-Gluc (5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid, cyclohexylammonium salt) is a substrate for β-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.
Detailní popis
Thermo Scientific X-Gluc (5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, cyclohexylammonium salt) is a substrate for beta-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate of chloro-bromoindigo (see the scheme below).
Applications
- Detection of GUS expression in plant cells and tissues (see Reference 1)
- Detection of infections caused by E. coli
- Detection of bacterial contamination in food and water samples.
Glucuronidase cleaves X-Gluc
Note
Preparation of a 0.1 M stock solution in dimethylformamide or dimethylsulfoxide is recommended.
| CategoryName | |
| Formula | C14H13BrClNO7 × C6N13N |
| Hazardous | No |
| Hazardous: | No |
| Molecular Weight | 521.8 |
| Quality Control | Purity of ≥ 97% confirmed by HPLC |
| Shelf Life: | |
| Shipping Condition: | |
| Storage Condition | -20 C |
| Storage Condition: | -20 C |
| Storage Note | Store at -20°C in the dark. |
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