WELQut Protease
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WELQut Protease is a highly specific serine protease cleaving outside the WELQX recognition motif to remove N-terminal protein fusion tags.
Detailní popis
Thermo Scientific WELQut Protease is highly specific, recombinant serine protease of Staphylococcus aureus. It recognizes and precisely cleaves recombinant proteins containing an engineered recognition sequence† W- E- L- Q↓X (Trp, Glu, Leu, Gln, X can be any amino acid). The protease cleaves outside the recognition sequence without leaving additional amino acids bound to the target protein.
The WELQut Protease is active in a broad temperature (4 to 30°C) and pH (pH 6.5 to 9.0) range and does not require specific buffers.
In addition, this new protease has several procedural advantages - it is ideal for on-column proteolysis reactions and can be easily removed from reaction mixtures using its built-in His-tag.
Highlights
- Cleaves outside WELQ recognition sequence, without leaving additional amino acids bound to the target protein
- Highly specific to cognate recognition site, does not generate non-specific product bands, even after long incubation and using excess of protease
- Easy to remove from the reaction mixture using built-in His-tag
- Ideal for on-column proteolysis reactions
Applications
- Removal of N-terminal fusion tags from recombinant protein preparations.
Footnote
† This cleavage sequence is present in expression vector pLATE52 included into the Thermo Scientific aLICator LIC Cloning and Expression Kit 4 (N-terminal His-tag/WELQut) (#K1281) available from Thermo Scientific.
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| Definition of Activity Unit | Each unit is defined as the amount of enzyme required to cleave ≥ 99% of 100 µg of a control protein in 16 h at 20°C. Enzyme activity is assayed in 100 µL 100 mM Tris-HCl (pH 8.0) |
| Hazardous | No |
| Hazardous: | No |
| Molecular Weight | 22 kDa monomer |
| Shelf Life: | |
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| Shipping Information | |
| Source | Bacillus subtilis cells with a cloned gene of SplB protease from Staphylococcus aureus |
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| Storage Buffer | Enzyme is supplied in 10 mM Na2HPO4; 1.8 mM KH2PO4, pH 7.3; 140 mM NaCl; 2.7 mM KCl, 50% glycerol |
| Storage Condition | -20 C |
| Storage Condition: | -20 C |
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