TrueStart Hot Start Taq DNA Polymerase

Thermo Scientific TrueStart Hot Start Taq DNA Polymerase is designed for hot start PCR, a technique shown to improve specificity, sensitivity and yield of DNA amplification during PCR (see References 1,2,3,4,5). TrueStart Hot Start Taq DNA Polymerase is chemically modified by adding proprietary heat-labile blocking groups to amino acid residues. TrueStart Hot Start Taq DNA Polymerase is inactive at room temperature because of this modification, avoiding extension of non-specifically annealed primers or primer dimers.

TrueStart Hot Start Taq DNA Polymerase activates rapidly during the initial denaturation step of PCR. The lack of additional activation step differentiates it from other hot start DNA polymerases, making this enzyme an ideal tool for both automatic hot start PCR and fast PCR. Activated TrueStart Hot Start Taq DNA Polymerase catalyzes 5'→3' synthesis of DNA, lacks detectable 3'→5' exonuclease (proofreading) activity, but possesses low 5'→3' exonuclease activity. These two activities are not detectable before activation.

Highlights

• Fastest activating hot start Taq DNA polymerase
• High PCR specificity – reduced effects of mis-priming and primer-dimer formation
• Enhanced PCR sensitivity
• Convenient room temperature PCR set-up
• Generates PCR products with 3’-dA overhangs

Applications

• High throughput hot start PCR
• Highly specific amplification of genomic and cDNA targets up to 3 kb
• Amplification of low copy DNA targets
• Multiplex PCR

Includes

• TrueStart Hot Start Taq DNA Polymerase (5 U/µL)
• 10X TrueStart Taq Buffer
• 25 mM MgCl₂