Thermo Scientific PageRuler Unstained High Range Protein Ladder

Detailní popis

The Thermo Scientific PageRuler Unstained High Range Protein Ladder is a  mixture of 8 unstained proteins (60 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver (see References 1-2). The 150 kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue, or other protein stains (see References 2-3). In addition, the ladder proteins contain an integral Step-tag II Sequence and may be detected on Western blots using Strep-Tactin Conjugates. The PageRuler Unstained High Range Protein Ladder is supplied ready-to-use: no heating, dilution, or addition of a reducing agent is required before use.

Highlights

• Size range – 8 proteins spanning 60 to 250 kDa
• Ready-to-use – supplied in a loading buffer for direct loading on gels
• Sharp bands – excellent accuracy for detection with coomassie or silver stains
• Reference band – 150 kDa band has greater intensity for easy orientation
• Tagged – each protein in the ladder contains an integral Strep-tag II Sequence
• Quality tested – each lot evaluated by SDS-PAGE and Western blotting

Applications

• Accurate protein sizing on SDS-polyacrylamide gels and Western blots

  Bands	250, 200, 150, 120, 100, 85, 70, and 60 kDa
  Content	Proteins (0.02 to 0.05 mg/mL of each) in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue, and 33% glycerol
  Hazardous	No