Taq DNA Polymerase (native, with BSA)
Low concentration available Store at -20°C Not inactivated at 80°C in 20 min
Highly thermostable, native DNA polymerase for routine PCR.
Detailní popis
Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E. coli.
Taq DNA Polymerase (native, with BSA) is supplied with BSA as a stabilizing agent. This version of Taq DNA Polymerase is often the best choice when amplifying DNA samples of lower purity, e.g. genomic DNA from mouse tail.
Highlights
- Thermostable – half life is more than 40 min at 95°C.
- Generates PCR products with 3’-dA overhangs.
- Supplied with two buffers – 10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).
Applications
- Routine PCR amplification of DNA fragments up to 5 kb
- DNA labeling (see References 1-3)
- High throughput PCR
Includes
Taq DNA Polymerase (native, with BSA):
- Taq DNA Polymerase 5 U/µL
- 10X Taq Buffer with KCl
- 10X Taq Buffer with (NH4)2SO4
- 25 mM MgCl2
- 20 mg/mL BSA
Taq DNA Polymerase (native, without BSA):
- Taq DNA Polymerase 5 U/µL
- 10X Taq Buffer with KCl
- 10X Taq Buffer with (NH4)2SO4
- 25 mM MgCl2
Taq DNA Polymerase (native, without BSA), LC:
- Taq DNA Polymerase 1 U/µL
- 10X Taq Buffer with KCl
- 10X Taq Buffer with (NH4)2SO4
- 25 mM MgCl2
Note
- The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in (see Reference 4). Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
- The 10X Taq Buffer without Detergent is recommended for microarray experiments.
10X Taq Buffer with (NH4)2SO4 | 750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM (NH4)2SO4, 0.1% (v/v) Tween 20. |
10X Taq Buffer with KCl | 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% (v/v) Nonidet P40. |
Definition of Activity Unit |
|
Hazardous | No |
Inactivation | Inactivated by phenol/chloroform extraction. |
Inhibition | Inhibitors: ionic detergents (deoxycholate, sarkosyl and SDS) at concentrations higher than 0.06, 0.02, and 0.01%, respectively (5). |
Molecular Weight | 94 kDa monomer |
Quality Control |
|
Source | Thermus aquaticus YT1 cells |
Storage Buffer | The enzyme is supplied in 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20 and 50% (v/v) glycerol. |
Storage Condition | -20 C |
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