T4 RNA Ligase

Kód produktu: EL0021 Kód výrobce: EL0021 Kód dodavatele: {DBB2285F-320D-415F-B0EF-EEDCE7C4ABC7} Výrobce: UAB Fermentas
1 815,00 Kč
1 500,00 Kč bez DPH
do týdne
1000 units
LO certified
Recombinant enzyme
Thermal inactivation at 70°C in 10 min
T4 RNA Ligase catalyzes the ATP-dependent formation of phosphodiester bonds between 5'-P and 3'-OH termini of oligonucleotides, single-stranded RNA and DNA.
Zobraz detailní popis

Detailní popis

Thermo Scientific T4 RNA Ligase catalyzes the ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA.

The minimal substrate is a nucleoside 3',5'-biphosphate in intermolecular reaction and oligonucleotide of 8bases in intramolecular reaction.

Applications

  • RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate (seeReference1)
  • Joining RNA to RNA(seeReference2)
  • Synthesis of oligoribonucleotides and oligodeoxyribonucleotides(seeReferences 3, 4)
  • Specific modifications of tRNAs(seeReference5)
  • Oligodeoxyribonucleotide ligation to single-stranded cDNAs for 5' RACE (Rapid Amplification of cDNA Ends)(seeReference6)
  • Site-specific generation of composite primers for PCR(seeReference7)

Note

The recommended BSA concentration in the reaction mixture is 0.1mg/mL.

10X Reaction Buffer 500mM Tris-HCl (pH7.5 at 25°C), 100mM MgCl2, 100mM DTT, 10mM ATP
CategoryName
Concentration 10 U/µL
Definition of Activity Unit
  • One unit of the enzyme catalyzes the conversion of 1nmol of 5'-[32P]-(A)12-18 to a phosphatase-resistant form in 30min at 37°C.
  • Enzyme activity is assayed in the following mixture: 50mM Tris-HCl (pH7.5), 10mM MgCl2, 10mM DTT, 1mM ATP, 10µM 5'-[32P]-(A)12-18 (10µM in 5'-termini).
Hazardous No
Hazardous: No
Inactivation Inactivated by heating at 70°C for 10min.
Inhibition Inhibitors: metal chelators, SH group-modifying reagents (8)
Molecular Weight 43.6kDa monomer
Quality Control The absence of ribonucleases, exodeoxyribonucleases, endodeoxyribonucleases, and phosphatases confirmed by appropriate quality tests.
Shelf Life:
Shipping Condition:
Shipping Information
Source E.coli cells with a cloned gene 63 of bacteriophage T4
SpecificationName
SpecificationValue
Storage Buffer The enzyme is supplied in 20mM Tris-HCl (pH7.5), 1mM DTT, 50mM KCl, 0.1mM EDTA, 0.03%(v/v) ELUGENT Detergent and 50%(v/v) glycerol.
Storage Condition -20 C
Storage Condition: -20 C

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