Thermo Scientific Transcription Promoter Sequencing Primers are single-stranded oligonucleotides with 5'- and 3'-hydroxyl ends. The primers are complementary to the SP6, T3, or T7 RNA polymerase promoter regions respectively. Primers are supplied as 10 µM aqueous solutions.
- Sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in common cloning vectors, such as pTZ19R, pTZ57R, and pBluescript II
Functionally tested by dideoxy sequencing of a vector containing an appropriate RNA polymerase promoter sequence.
|SO116||SP6 promoter sequencing primer, 18-mer
|SO117||SP6 promoter sequencing primer, 24-mer
|SO118||T7 promoter sequencing primer, 20-mer
|SO119||T3 promoter sequencing primer, 17-mer
|SO120||T3 promoter sequencing primer, 24-mer
Primers cannot be used for certain plasmids that contain truncated, but still fully functional promoters. Primers are not phosphorylated.
|Storage Condition||-20 C|
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