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Potvrzuji
  1. Úvodní strana
  2. Reagencie
  3. Life Technologies / Fermentas
  4. DNA/RNA modifying enzymes
  5. Ribonucleases (RNases)
  6. RNase T1

RNase T1

Kód produktu: EN0541 Kód výrobce: EN0541 Kód dodavatele: {004389A5-DD61-46CD-87F8-2DBDD2FF4531} Výrobce: Life Technologies Czech Republic s.r.o.
RNase T1
2 366,76 Kč
1 956,00 Kč bez DPH
do týdne
100000 u
RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.
Zobraz detailní popis

Detailní popis

Thermo Scientific RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues (see Figure 1 in Supporting Data). It cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleotides with the formation of corresponding intermediate 2', 3'-cyclic phosphate (see Reference 1). The reaction products are 3'-GMP and oligonucleotides with a terminal 3'-GMP.

RNase T1 does not require metal ions for activity.

Applications

  • Removal of RNA from DNA preparations
  • RNA sequencing (see​ Reference 1)
  • Ribonuclease protection assays. Used in conjunction with RNase A (see​ Reference 2)
  • Removal of RNA from recombinant protein preparations
  • Determination of the level of RNA transcripts synthesized in vitro from DNA templates containing a "G-less cassette" (see​ Reference 3)
CategoryName
Concentration 1000 U/µL
Definition of Activity Unit One unit of the enzyme causes an increase in absorbance of 1.0 at 260 nm in 15 min when yeast RNA is hydrolyzed at 37°C and pH 7.5.
Hazardous No
Hazardous: No
Inactivation Inactivation by heating is reversible, reliably removed by spin column or phenol/chloroform extraction.
Inhibition Inhibitors: metal ions Mg2+, Ca2+, Zn2+, Fe2+, Cu2+ (MgCl2 at 100 mM concentration is approx. 40% inhibitory, CaCl2 at 10 mM is approx. 30% inhibitory); mononucleotides (2'-GMP, 3'-GMP, etc.); guanilyl-2',5'-guanosine is a specific inhibitor (see Reference 4).
Molecular Weight 11.2 kDa monomer.
Quality Control
  • The absence of endo-, exodeoxyribonucleases, and proteases confirmed by appropriate quality tests.
  • Functionally tested for RNA digestion in the plasmid DNA purification procedure (in conjunction with RNase A).
Shelf Life:
Shipping Condition:
Shipping Information
Source E. coli cells with a cloned rntA gene of Aspergillus oryzae.
SpecificationName
SpecificationValue
Storage Buffer The enzyme is supplied in 50 mM Tris-HCl (pH 7.4) and 50% (v/v) glycerol.
Storage Condition -20 C
Storage Condition: -20 C

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