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Potvrzuji
  1. Úvodní strana
  2. Reagencie
  3. Life Technologies / Fermentas
  4. DNA/RNA modifying enzymes
  5. Ribonucleases (RNases)
  6. RNase I

RNase I

Kód produktu: EN0602 Kód výrobce: EN0602 Kód dodavatele: {6384EC14-C64A-4325-87D6-B2BE5D86F162} Výrobce: Life Technologies Czech Republic s.r.o.
RNase I
12 608,20 Kč
10 420,00 Kč bez DPH
do týdne
5000 units
Ribonuclease I (RNase I) is an endoribonuclease that hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates.
Zobraz detailní popis

Detailní popis

Thermo Scientific Ribonuclease I (RNase I), an endoribonuclease, preferentially hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates via nucleoside 2', 3'-cyclic monophosphate intermediates (see Figure 1 in Supporting Data [see Reference 1]).

The enzyme does not require any metal ions for activity.

Highlights

  • Stable and active under a wide variety of reaction conditions
  • Can be heat inactivated in 30 minutes at 100°C

Applications

  • Removal of RNA from DNA solutions (see Reference 2)
  • Removal of RNA from recombinant protein preparations
  • Ribonuclease protection assays (see Reference 3)

Notes

Mammalian ribonuclease inhibitors have no effect on RNase I. RNase I  binds to DNA, but does not degrade it. RNase I amino acid sequence is typical for the RNase T2 family. Non-ionic detergents (e.g., Triton X-100) do not inhibit RNase I, and may even slightly stimulate its activity and stabilize it against heat inactivation. Triton X-100 or BSA (at 0.1 mg/mL) may prevent RNase I from sticking to glass vessels when working with dilute solutions. Polyamines stimulate the activity of RNase I.

CategoryName
Concentration 10 U/µL
Definition of Activity Unit One unit of the enzyme catalyzes degradation of 100 ng of E. coli ribosomal RNA per second into acid-soluble nucleotides at 37°C. Enzyme activity is assayed in the following mixture: 20 mM Tris-acetate (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 0.01% (v/v) Triton X-100, 40 µM E. coli ribosomal [3H]-RNA.
Hazardous No
Hazardous: No
Inactivation Inactivated by heating at 100°C for 30 min, reliably removed by spin column or phenol/chloroform extraction.
Inhibition Inhibitors: SDS at 0.1% concentration irreversibly inactivates the enzyme.
Molecular Weight 29.6 kDa monomer
Quality Control The absence of endo- and exodeoxyribonucleases confirmed by appropriate quality tests.
Shelf Life:
Shipping Condition:
Shipping Information
Source E. coli cells with a cloned rna gene of E.coli.
SpecificationName
SpecificationValue
Storage Buffer The enzyme is supplied in 50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.01% (v/v) Triton X-100, and 50% (v/v) glycerol.
Storage Condition -20 C
Storage Condition: -20 C

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