RNase H
Kód produktu: EN0201
Kód výrobce: EN0201
Kód dodavatele: {6A6430BB-4352-4E23-853D-2E28D6FF8B39}
Výrobce:
Life Technologies Czech Republic s.r.o.
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Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.
Detailní popis
Thermo Scientific Ribonuclease H (RNase H) specifically degrades the RNA strand in RNA-DNA hybrids (see Figure 1 in Supporting Data). It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.
Applications
- Removal of mRNA prior to synthesis of second strand cDNA (see Reference 1)
- RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis
- Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT) (see Reference 2)
- Site-specific cleavage of RNA (see Reference 3)
- Studies of in vitro polyadenylation reaction products (see Reference 4)
| 10X Reaction Buffer | 200 mM Tris-HCl (pH 7.8), 400 mM KCl, 80 mM MgCl2, 10 mM DTT |
| Concentration | 5 U/µL |
| Definition of Activity Unit | One unit of the enzyme catalyzes the formation of 1 nmol of acid soluble products in 20 min at 37°C. Enzyme activity is assayed in the following mixture: 20 mM Tris-HCl (pH 7.8), 40 mM KCl, 8 mM MgCl2, 1 mM DTT, 24 µM [3H]-poly(A)·poly(dT), 0.03 mg/mL BSA, 4% (v/v) glycerol. |
| Hazardous | No |
| Inactivation | Inactivated by heating at 65°C for 10 min. |
| Inhibition | Inhibitors: metal chelators, SH-blocking reagents. |
| Molecular Weight | 18.4 kDa monomer |
| Quality Control | The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests. |
| Source | E.coli MRE-600 cells |
| Storage Buffer | The enzyme is supplied in: 25 mM HEPES-KOH (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1 mg/mL BSA, and 50% (v/v) glycerol. |
| Storage Condition | -20 C |
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