RiboRuler™ Low Range RNA Ladder
RiboRuler Low Range RNA Ladder is a mixture of 7 chromatography purified single-stranded RNA transcripts (100 to 1000 bp long), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments.
Detailní popis
Thermo Scientific RiboRuler Low Range RNA Ladder 100-1000 bp is a mixture of 7 chromatography purified single-stranded RNA transcripts (100-1000 bp long), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments.
Ladder is available in 1 mM EDTA, pH 6.0.
RiboRuler Low Range RNA Ladder is composed of chromatography-purified transcripts what makes them free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically (see Photo for precise quantities). To minimize the possibility of contamination during the use the ladder is aliquotted in smaller volumes.
RiboRuler Low Range RNA Ladder is optimal for end-labeling using T4 Polynucleotide Kinase (see Protocol) and is ideal for Northern blots.
Highlights
- Sharp bands of uniform intensity
- Easy-to-remember band sizes and quantities
- Approximate RNA quantification by predetermined concentration of ladder bands
- Supplied with 2X RNA Loading Dye
- Stable for 6 months at -20°C
Applications
- RNA sizing and quantification on native or denaturing gels
- Northern blotting.
| 2X RNA Loading Dye | 95% formamide, 0.025% SDS, 0.025% bromophenol blue, 0.025% xylene cyanol FF, 0.025% ethidium bromide, and 0.5 mM EDTA. |
| Hazardous | No |
| Quality Control |
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| Storage | Store at -20°C for 6 months or at -70°C for longer periods. |
| Storage Buffer | 1 mM EDTA (pH 6.0). |
| Storage Condition | -20 C |
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