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Potvrzuji
  1. Úvodní strana
  2. Reagencie
  3. Life Technologies / Fermentas
  4. Molecular Cloning
  5. Generation of DNA fragments for cloning
  6. RiboLock™ RNase Inhibitor

RiboLock™ RNase Inhibitor

Kód produktu: EO0384 Kód výrobce: EO0384 Kód dodavatele: {20C9A24F-C3FB-45CE-B70F-79719D262E39} Výrobce: Life Technologies Czech Republic s.r.o.
RiboLock™ RNase Inhibitor
38 296,50 Kč
31 650,00 Kč bez DPH
do týdne
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RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions
Zobraz detailní popis

Detailní popis

Thermo Scientific RiboLock RNase Inhibitor inhibits the activity of RNases A (see Figure 1 in Supporting Data), B, and C by binding them in a  noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H.

Highlights

  • Performs under a wide range of reaction conditions
  • Protects RNA from degradation at temperatures up to 55°C (see Figure 2 in Supporting Data)

Note

DTT provided in the Storage Buffer ensures stability during long term storage, but is not necessary for inhibitor activity. Recommended concentration 1 U/µL of a reaction mixture.

Concentration 40 U/µL
Definition of Activity Unit
  • One unit of the RiboLock RNase Inhibitor inhibits the activity of 5 ng RNase A by 50%.
  • Inhibitor activity is assayed in the following mixture: 100 mM Tris-HCl (pH 7.5), 1.2 mM EDTA, 0.1 mg/mL BSA, 100 ng/mL RNase A, 0.1 mg/mL [3H]-RNA, 50 mg/mL yeast RNA, 8 mM DTT.
Hazardous No
Inactivation Inactivated by heating at 75°C for 10 min. Residual activity detectable after 10 min heating at 70°C.
Inhibition Inhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RiboLock RNase Inhibitor and release the RNase bound.
Molecular Weight 49.6 kDa monomer
Quality Control
  • The absence of ribonucleases, endo-, exodeoxyribonucleases and phosphatases confirmed by appropriate quality tests.
  • Functionally tested in RNA and cDNA synthesis.
Source E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor.
Storage Buffer The enzyme is supplied in 20 mM HEPES-NaOH (pH 7.5), 50 mM NaCl, 8 mM DTT, 0.03% v/v) ELUGENT Detergent and 50% (v/v) glycerol.
Storage Condition -20 C

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