RiboLock™ RNase Inhibitor
Thermo Scientific RiboLock RNase Inhibitor inhibits the activity of RNases A (see Figure 1 in Supporting Data), B, and C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H.
- Performs under a wide range of reaction conditions
- Protects RNA from degradation at temperatures up to 55°C (see Figure 2 in Supporting Data)
DTT provided in the Storage Buffer ensures stability during long term storage, but is not necessary for inhibitor activity. Recommended concentration 1 U/µL of a reaction mixture.
|Definition of Activity Unit||
|Inactivation||Inactivated by heating at 75°C for 10 min. Residual activity detectable after 10 min heating at 70°C.|
|Inhibition||Inhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RiboLock RNase Inhibitor and release the RNase bound.|
|Molecular Weight||49.6 kDa monomer|
|Source||E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor.|
|Storage Buffer||The enzyme is supplied in 20 mM HEPES-NaOH (pH 7.5), 50 mM NaCl, 8 mM DTT, 0.03% v/v) ELUGENT Detergent and 50% (v/v) glycerol.|
|Storage Condition||-20 C|
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