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Potvrzuji
  1. Úvodní strana
  2. Reagencie
  3. Life Technologies / Fermentas
  4. DNA/RNA modifying enzymes
  5. DNA/RNA Polymerases
  6. Properties of reverse transcriptases
  7. RevertAid Reverse Transcriptase

RevertAid Reverse Transcriptase

Kód produktu: EP0442 Kód výrobce: EP0442 Kód dodavatele: {22473C15-716A-4EDB-833D-928B6286AD13} Výrobce: Life Technologies Czech Republic s.r.o.
RevertAid Reverse Transcriptase
10 527,00 Kč
8 700,00 Kč bez DPH
do týdne

A genetically modified M-MuLV RT for routine performance in reverse transcription.

Zobraz detailní popis

Detailní popis

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase (see References 1, 2).

Highlights

  • Efficient synthesis of full-length first strand cDNA up to 13 kb
  • Optimum activity at 42°C
  • Active up to 50°C
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)

Applications

  • First strand cDNA synthesis for RT-PCR and real-time RT-PCR
  • Synthesis of cDNA for cloning and expression
  • Generation of labeled cDNA probes for microarrays
  • DNA labeling (see Reference 3)
  • Analysis of RNA by primer extension (see​ Reference 3)

Includes

  • RevertAid Reverse Transcriptase (200 U/µL)
  • 5X Reaction Buffer

Note

Also available: RevertAid First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase (see References 1, 2).

Highlights

  • Efficient synthesis of full-length first strand cDNA up to 13 kb
  • Optimum activity at 42°C
  • Active up to 50°C
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)

Applications

  • First strand cDNA synthesis for RT-PCR and real-time RT-PCR
  • Synthesis of cDNA for cloning and expression
  • Generation of labeled cDNA probes for microarrays
  • DNA labeling (see Reference 3)
  • Analysis of RNA by primer extension (see​ Reference 3)

Includes

  • RevertAid Reverse Transcriptase (200 U/µL)
  • 5X Reaction Buffer

Note

Also available: RevertAid First Strand cDNA Synthesis Kit

Molecular Weight 76.1 kDa monomer
5x Reaction Buffer 250 mM Tris-HCl (pH 8.3 at 25°C), 250 mM KCl, 20 mM MgCl2, 50 mM DTT
Definition of Activity Unit
  • One unit of the enzyme incorporates 1 nmol of dTMP into a polynucleotide fraction (adsorbed on DE-81) in 10 min at 37°C.
  • Enzyme activity is assayed in the following mixture: 50 mM Tris-HCl (pH 8.3), 4 mM MgCl2, 10 mM DTT, 50 mM KCl, 0.5 mM dTTP, 0.4 MBq/mL [3H]-dTTP, 0.4 mM polyA·oligo(dT)12-18.
Hazardous No
Inactivation Inactivated by heating at 70°C for 10 min.
Inhibition Metal chelators, inorganic phosphate, pyrophosphate and polyamines (see Reference 2)
Quality Control
  • The absence of endo-, exodeoxyribonucleases, phosphatases, and ribonucleases confirmed by appropriate quality tests.
  • Functionally tested in first strand cDNA synthesis and RT-PCR.
RevertAid Reverse Transcriptase (200 U/µL) is supplied in 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, and 50% (v/v) glycerol
Source E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.
Storage Condition -20 C

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