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5'...R G↓G W C C Y...3'
3'...Y C C W G↑G R...5'
Available as a FastDigest enzyme for rapid DNA digestion
The Psp5II (PpuMI) restriction enzyme recognizes RG^GWCCY sites and cuts best at 37°C in G buffer (Isoschizomers: PpuMI, PspPPI)
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5'...A A T↓A T T...3'
3'...T T A↑T A A...5'
Available as a FastDigest enzyme for rapid DNA digestion
The SspI restriction enzyme recognizes AAT^ATT sites and cuts best at 37°C in G buffer
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B buffer for 100% activity FastDigest buffer for 100% activity G buffer for 100% activity O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 75°C in 10 min
T4 DNA Polymerase T4 DNA Polymerase is template-dependent and catalyzes the 5'-3' synthesis from primed single-stranded DNA.
The GeneJET Plasmid Miniprep Kit is a simple, rapid, and cost-effective system for the isolation of high quality plasmid DNA from recombinant E. coli cultures.
The GeneJET Plasmid Miniprep Kit is a simple, rapid, and cost-effective system for the isolation of high quality plasmid DNA from recombinant E. coli cultures.