Rapid CM DNA Lib Prep kit na 24rxns ( NGS knihovny)

The Rapid CM DNA Lib Prep kit provides a streamlined and efficient method to construct DNA library for next generation sequencing (NGS) with Illumina® platforms. The entire protocol can be completed in 2 hours. Starting from fragmented input-DNA (1 - 1000 ng), the kit contains all the enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, the fragmented DNA is end-repaired and adenylated during the initial step. The kit can be conveniently used for construction of PCR-free libraries from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in the library amplification.

The following workflow illustrates the processes of the Rapid DNA Lib Prep Kit.

	Tube name	8 RXN	24 RXN	96 RXN
End Preparation	End Prep Buffer	100 μl	260 μl	1000 μl
	End Prep Enzymes CM	30 μl	78 μl	300 μl
Adaptor Ligation	Ligase MM	165 μl	429 μl	1650 μl
	Ligase Mix CM	30 μl	78 μl	300 μl
	Low-EDTA TE Buffer	1000 μl	2 mL	5 mL
Amplification	2X PCR Mix	250 μl	650 μl	2.5 mL
	10X PCR Primers	40 μl	120 μl	500 μl

The Low-EDTA TE buffer can be stored at room temperature or 4°C.
All other components should be stored at -20°C.
The shelf lives of all reagents are one year when stored properly.

Mechanical or enzymatic methods for DNA fragmentation
Multiplex adapters (cat. no. RK20282, RK20283, RK20284, RK20285, RK20292, RK20293, RK20294, RK20295) compatible with Illumina® platforms
100% ethanol (ACS grade)
Nuclease-free water
PCR strip tubes or plates
Magnetic stand
Thermocycler
AgencourtTM AMPure XP bead (Beckman Coulter Inc., cat. no. A63880)
Pipettes and multichannel pipettes
Aerosol resistant pipette tips
Microcentrifuge
Vortex mixer
Agilent Bioanalyzer or comparable method to assess the quality of DNA library