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Potvrzuji
  1. Úvodní strana
  2. Reagencie
  3. Life Technologies / Fermentas
  4. In vitro TRANSCRIPTION
  5. High yield in vitro transcriptio(up to 200µg RNA per 20µl reaction)
  6. Pyrophosphatase, Inorganic

Pyrophosphatase, Inorganic

Kód produktu: EF0221 Kód výrobce: EF0221 Kód dodavatele: {B7609369-87B2-408B-8D77-7C31B030C220} Výrobce: Life Technologies Czech Republic s.r.o.
Pyrophosphatase, Inorganic
1 955,36 Kč
1 616,00 Kč bez DPH
do týdne
10 Unit
LO certified Not inactivated at 80°C in 20 min Recombinant enzyme
Pyrophosphatase, Inorganic catalyzes the hydrolysis of inorganic pyrophosphate to two orthophosphates.
Zobraz detailní popis

Detailní popis

Thermo Scientific Pyrophosphatase, Inorganic catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates (see Figure 1 in Supporting data). The enzyme requires a divalent metal cation with Mg2+ conferring the highest activity (see Reference 1).

Highlights

  • Active in Thermo Scientific buffers for DNA polymerases, RNA polymerases, and reverse transcriptases 

Applications

  • High yield RNA synthesis by in vitro transcription (see Reference 2)
  • DNA polymerization reactions: preventing accumulation of pyrophosphate (see References 3, 4)
  • Removal of contaminant PPi in reagents used for SNP genotyping by methods based on the detection of pyrophosphate (see Reference 5)

Note

The enzyme can be diluted with the supplied storage (dilution) buffer. Use of this enzyme in certain applications may be covered by patents and may require a license.

CategoryName
Concentration 0.1 u/µL
Definition of Activity Unit
  • One unit of the enzyme hydrolyzes 1 µmol of inorganic pyrophosphate in 1 minute at 25°C.
  • Enzyme activity is assayed in the following mixture: 100 mM Tris-HCl (pH 7.2), 2 mM MgCl2 and 2 mM inorganic pyrophosphate (PPi).
Hazardous No
Hazardous: No
Inhibition and Inactivation
  • Inhibitors: imidodiphosphate, alpha, omega-glycol disphosphates, methanedial diphosphate, 1,2-ethanedial diphosphate (see Reference 6).
  • Inactivation by heating is not complete, reliably removed by spin column or phenol/chloroform extraction.
Molecular Weight This enzyme is a homodimer. It consists of two identical subunits of 32 kDa.
Quality Control The absence of endo-, exodeoxyribonucleases, and ribonucleases confirmed by appropriate quality tests.
Shelf Life:
Shipping Condition:
Shipping Information
Source E. coli cells with a cloned ppa gene of Saccharomyces cerevisiae.
SpecificationName
SpecificationValue
Storage (Dilution) Buffer The enzyme is supplied in: 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA and 50% (v/v) glycerol.
Storage Condition -20 C
Storage Condition: -20 C

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