pUC57 DNA
Kód produktu: SD0171
Kód výrobce: SD0171
Kód dodavatele: {9A4C9160-6C67-4262-A540-B3BEF3F41841}
Výrobce:
Life Technologies Czech Republic s.r.o.
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. Thermo Scientific Plasmid pUC57, 2710 bp in length, is a derivative of pUC19. pUC57 MCS contains 6 restriction sites with protruding 3’-ends that are resistant to E. coli exonuclease III. This vector is designed for cloning and generation of ExoIII deletions. The exact positions of the genetic elements are shown on the map (termination codons included). DNA replication initiates at position 890 (±1) and proceeds in the direction indicated. The bla gene nucleotides 2510-2442 (compl. strand) code for a signal peptide.
Detailní popis
Highlights
- More than 90% in the supercoiled form
- Isolated from E. coli (dam+, dcm+)
- Purified by chromatography using proprietary patented technology
Applications
- Cloning
- Sequencing of insert DNA
- Generation of nested deletions with ExoIII
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