Proteinase K (recombinant), PCR grade
Kód produktu: EO0491
Kód výrobce: EO0491
Kód dodavatele: {AF206C29-C900-444E-B574-B14445ED5531}
Výrobce:
Life Technologies Czech Republic s.r.o.
![Recombinant enzyme Recombinant enzyme](http://www.thermoscientificbio.com/uploadedImages/Products/DNA_RNA_Modifying_Enzymes/Other_Enzymes/recomb.gif)
Proteinase K is a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations. It degrades proteins even in the presence of detergents.
Detailní popis
Thermo Scientific Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic, or hydrophobic amino acids. The Proteinase K is classified as a serine protease (see Reference 1). The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.
Highlights
- Ready-to-use solution
- Active in a wide range of reaction conditions
Applications
- Isolation of genomic DNA from mouse tail
- Isolation of genomic DNA from cultured cells
- Removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines (see References 2, 3)
- Determination of enzyme localization (see Reference 4)
- Improving cloning efficiency of PCR products (see Reference 5)
Note
- The recommended working concentration of Proteinase K is 0.05 to 1 mg/mL. The activity of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to 4 M urea (see Reference 3)
- Ca2+ protects Proteinase K against autolysis, increases the thermal stability, and has a regulatory function for the substrate binding site of Proteinase K (see Reference 7)
- Stable over a wide pH range: 4.0 to 12.5, optimum pH 7.5 to 8.0 (see Reference 8)
- Optimum activity at 50 to55°C
- Rapid denaturation of enzyme occurs at temperatures above 65°C
Definition of Activity Unit |
|
Hazardous | No |
Inhibition |
|
Molecular Weight | 28.9 kDa monomer (see Reference 6) |
Quality Control | The absence of endo-, exodeoxyribonucleases, and ribonucleases confirmed by appropriate quality tests. |
Source | Pichia pastoris cells with a cloned gene encoding Tritirachium album endolytic protease (Proteinase K). |
Storage Buffer | The enzyme is supplied in: 10 mM Tris-HCl (pH 7.5), containing calcium acetate and 50% (v/v) glycerol. |
Storage Condition | -20 C |
Hodnocení produktu
Produkt zatím nikdo nehodnotil, buďte první!