The Thermo Scientific Pierce Prestained Protein Molecular Weight Marker is a mixture of six blue-stained natural proteins (20 to 120 kDa) for use as size standards in protein electrophoresis (SDS-PAGE).
This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins (1-3) that have been made visible with gel stains or Western blot detection reagents.
- Size range – six proteins spanning 20 to 120 kDa
- Ready-to-use – supplied in a loading buffer for direct loading on gels
- Prestained – for direct viewing during electrophoresis
- Quality tested – each lot evaluated by SDS-PAGE and Western blotting
- Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.
- Monitoring protein transfer onto membranes after Western blotting
- Sizing of proteins on SDS-polyacrylamide gels and Western blots
Hazardous No Quality Control Tested in SDS-polyacrylamide gel electrophoresis and Western Blotting. Storage Store at -20°C. Storage Buffer
- Dye-stained proteins in 62.5 mM Tris-HCl (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1.5 mM NaN3, and 33% glycerol
- Component proteins: beta-galactosidase (120 kDa), bovine serum albumin (85 kDa), ovalbumin (50 kDa), cardonic anhydrase (35 kDa), beta-lactoglobulin (25 kDa), lysozyme (20 kDa)
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