Prestained Protein MW Marker

Detailní popis

The Thermo Scientific Pierce Prestained Protein Molecular Weight Marker is a mixture of six blue-stained natural proteins (20 to 120 kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins (1-3) that have been made visible with gel stains or Western blot detection reagents.

Highlights

• Size range – six proteins spanning 20 to 120 kDa
• Ready-to-use – supplied in a loading buffer for direct loading on gels
• Prestained – for direct viewing during electrophoresis
• Quality tested – each lot evaluated by SDS-PAGE and Western blotting

Applications

• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.
• Monitoring protein transfer onto membranes after Western blotting
• Sizing of proteins on SDS-polyacrylamide gels and Western blots

  Hazardous	No
  Quality Control	Tested in SDS-polyacrylamide gel electrophoresis and Western Blotting.
  Storage	Store at -20°C.
  Storage Buffer	Dye-stained proteins in 62.5 mM Tris-HCl (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1.5 mM NaN3, and 33% glycerol Component proteins: beta-galactosidase (120 kDa), bovine serum albumin (85 kDa), ovalbumin (50 kDa), cardonic anhydrase (35 kDa), beta-lactoglobulin (25 kDa), lysozyme (20 kDa)