phiX174 RF1 DNA

Detailní popis

Thermo Scientific phiX174 was the first DNA virus discovered to have a  single-stranded, circular genome. phiX174 DNA is 5386 nt in length. The DNA strand packaged into the virion is termed the “plus” strand. After entering the cell, the Thermo Scientific phiX174 DNA is used as a  template for minus-strand synthesis, producing double-stranded DNA. The conversion of plus DNA strands to double-stranded DNA does not require any of the phage genes to function.

The double-stranded DNA can then be transcribed, resulting in synthesis of phage-encoded proteins. Synthesis of single-stranded (plus) DNA requires phage-encoded gene A protein. DNA synthesis is initiated at ori (+) and proceeds in the direction indicated. Late in infection, the single-stranded circles are encapsidated into new virions. The cycle terminates by cellular lysis, mediated by phage gene E encoded protein.

Highlights

• Purified by chromatography using proprietary patented technology
• More than 90% in the supercoiled form
• Isolated from E. coli HF4704 infected with phiX174 am3

Applications

• Preparation of DNA molecular weight standards

GenBank/EMBL Accession Numbers

V01128, J02482, M10348, M10379, M10714, M10749, M10750, M10866, M10867, M24859

ΦX174 DNA map. Download ΦX174 sequence in FASTA, GenBank, or EMBL format.

CategoryName
Hazardous	No
Hazardous:	No
Quality Control	DNA concentration is confirmed spectrophotometrically. Identity and homogenity of DNA is confirmed by digestion by BsuRI and HinfI, and analysis of DNA fragmentation patterns.
Shelf Life:
Shipping Condition:
Shipping Information
SpecificationName
SpecificationValue
Storage Buffer	10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.
Storage Condition	-20 C
Storage Condition: