phiX174 DNA/BsuRI (HaeIII) Marker, ready-to-use
Kód produktu: SM0253
Kód výrobce: SM0253
Kód dodavatele: {D4BF0361-CDB3-4E6E-81F2-0E61B66CD313}
Výrobce:
Life Technologies Czech Republic s.r.o.
Marker for sizing and approximate quantification of small double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels. Premixed with Loading Dye for direct loading onto gel.
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Detailní popis
phiX174 DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme, then purified and dissolved in storage buffer. The DNA fragments contain blunt or sticky ends, depending on the restriction enzyme used for the markers preparation.
Thermo Scientific phiX174 DNA/BsuRI (HaeIII) Marker, ready-to-use is premixed with 6X DNA Loading Dye for direct loading onto agarose and polyacrylamide gels.
Highlights
- Sizing and approximate quantification of small DNA fragments
- Sharp bands
- Premixed with 6X DNA Loading Dye for direct loading and room temperature storage
- Supplied with loading dye for sample DNA
Note
- Bands indicated** form an anomalous pattern on polyacrylamide gels.
- The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of the respective DNA sequences.
| 6X DNA Loading Dye | 10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA. |
| Hazardous | No |
| Quality Control |
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| Storage and Loading Buffer (for ready-to-use markers) | 10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 0.005% xylene cyanol FF, and 10% glycerol. |
| Storage Condition | -20 C |
| Storage Notes | Store at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C. |
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