phiX174 DNA/BsuRI (HaeIII) Marker
Kód produktu: SM0251
Kód výrobce: SM0251
Kód dodavatele: {40AA94C5-7CF7-479E-9DA5-1FDF2C93C3BB}
Výrobce:
Life Technologies Czech Republic s.r.o.
A conventional marker composed of 11 sharp bands for sizing and approximate quantification of small double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels.
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Detailní popis
Thermo ScientificphiX174 DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme, then purified and dissolved in storage buffer. The DNA fragments contain blunt or sticky ends, depending on the restriction enzyme used for the markers preparation.
Thermo Scientific phiX174 DNA/BsuRI (HaeIII) Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction, see protocols in Resources
Highlights
- Sizing and approximate quantification of small DNA fragments
- Sharp bands
- Supplied with loading dye for sample DNA
Note
- Bands indicated ** form an anomalous pattern on polyacrylamide gels.
- The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of the respective DNA sequences.
| 6X DNA Loading Dye | 10mM Tris-HCl (pH7.6), 0.03%bromophenol blue, 0.03%xylene cyanol FF, 60%glycerol, and 60mM EDTA. |
| Hazardous | No |
| Quality Control |
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| Storage Buffer (TE buffer) | 10mM Tris-HCl (pH7.6) and 1mM EDTA. |
| Storage Condition | -20 C |
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