PCR Master Mix (2X)

Detailní popis

Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5’→3’ synthesis of DNA, has no detectable 3’→5’ exonuclease (proofreading) activity and possesses low 5’→3’ exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.

PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Highlights

• Thermostable – half life is more than 40 min at 95°C
• Generates PCR products with 3’-dA overhangs
• Supplied with two buffers – 10X Taq Buffer with KCl and 10X Taq Buffer with (NH₄)₂SO₄. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)

Applications

• Routine PCR amplification of DNA fragments up to 5 kb
• High throughput PCR
• DNA labeling (see Reference 1,2,3)

Includes

Taq DNA Polymerase (recombinant):

• Taq DNA Polymerase 5 U/µL
• 10X Taq Buffer with KCl
• 10X Taq Buffer with (NH₄)₂SO₄
• 25 mM MgCl₂

Taq DNA Polymerase (recombinant), LC:

• Taq DNA Polymerase 1 U/µL
• 10X Taq Buffer with KCl
• 10X Taq Buffer with (NH₄)₂SO₄
• 25 mM MgCl₂

PCR Master Mix (2X):

• Taq DNA polymerase (0.05 U/µL), reaction buffer, 4 mM MgCl₂, and 0.4 mM of each dNTP
• Nuclease-free water

Note

• The error rate of Taq DNA Polymerase in PCR is 2.2 x 10^-5 errors per nt per cycle, as determined by a modified method described in (see Reference 4). Accordingly, the accuracy of PCR is 4.5 x 10⁴. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
• The 10X Taq Buffer without Detergent is recommended for microarray experiments.