PageRuler Prestained NIR Protein Ladder

Detailní popis

The Thermo Scientific PageRuler Prestained NIR Protein Ladder is a  mixture of 10 proteins (11 to 250 KDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing following electrophoresis.

The protein MW markers in this ladder resolve into sharp bands when analyzed by SDS-PAGE and are labeled with a fluorescent dye for visualization with instruments equipped for detection of near-infrared (NIR) fluorescence. The bands are also directly visible because the proteins are prestained blue. The 55 kDa band is of greater intensity and serves as a reference band. The protein ladder is conveniently packaged and ready to use: no heating, dilution, or addition of a  reducing agent is required before use.

Highlights

• Size range – 10 proteins spanning 11 to 250 kDa
• Fluorescent – detect using 670 nm red laser or 700 nm channels with fluorescence imagers
• Ready-to-use – supplied in a loading buffer for direct loading on gels
• Sharp bands – excellent sizing for near-infrared visualization of protein gels and blots
• Reference band – 55 kDa band has greater intensity for easy orientation
• Quality tested – each lot evaluated by SDS-PAGE and Western blotting

Applications

• Protein sizing on SDS-polyacrylamide gels and Western blots for visualization with near-infrared (NIR) fluorescence imagers

Requires

• Fluorescent imager capable of near-infrared fluorescence detection, such as certain Typhoon Imagers and the LI-COR Odyssey Infrared Imaging System

  Content	Proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, and 33% glycerol
  Hazardous	No