PageRuler Plus Prestained Protein Ladder
A prestained SDS-PAGE MW marker with contrasting colored reference bands at 10, 25, and 70 kDa.
Detailní popis
The Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of 9 blue-, orange-, and green-stained recombinant proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.
This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins (see References 1-3) that have been made visible with gel stains or Western blot detection reagents. A blue chromophore is bound to all proteins, except proteins of two reference bands of 70 kDa and 25 kDa that are colored with an orange dye and one green reference band of 10 kDa. PageRuler Plus Prestained Protein Ladder is ready to use: no heating, further dilution, or addition of a reducing agent is required before loading onto a gel.
Highlights
- Size range – nine proteins spanning 10 to 250 kDa
- Ready-to-use – supplied in a loading buffer for direct loading on gels, no need to boil
- Sharp bands – color-coded bands of similar intesity for easy visualization
- Quality tested – each lot evaluated by SDS-PAGE and Western blotting
- Bright reference bands – orange at 70 and 25 kDa, and green at 10 kDa
- Membrane-compatible – colored bands transfer to membranes for Western blotting
Applications
- Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
- Monitoring protein transfer onto membranes after Western blotting
- Sizing of proteins on SDS-polyacrylamide gels and Western blots
Note
For migration patterns under different gel conditions, see Specifications.
| Hazardous | No |
| Quality Control | Tested in SDS-polyacrylamide gel electrophoresis and Western Blotting. |
| Storage | Stable at 4°C for up to 3 months. For long term storage, store at -20°C. |
| Storage Buffer | 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, and 33% glycerol. |
Migration patterns of PageRuler Prestained Protein Ladder in different electrophoretic conditions
The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.
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