Thermo Scientific O'RangeRuler 10 bp DNA Ladder 10-150 bp is designed for precise sizing of PCR products and other double-stranded DNA fragments in agarose or non-denaturing polyacrylamide gels. It is a step ladder with 10 bp differences between the DNA fragments and consists of purified and ligated blunt-ended basic unit repeats of 10 bp. This DNA ladder is not recommended for DNA quantification.
O'RangeRuler 10 bp DNA Ladder is supplied with 6X Orange DNA Loading Dyefor sample DNA. Orange G dye can be used to monitor DNA migration in agarose or polyacrylamide gels and migrates at 50 bp in a 1% agarose gel. It is the preferable dye when visualization of small DNA fragments is important.
- Step ladder with 10 bp increments
- Sharp bands, easy-to-remember fragment sizes
- Evenly spaced reference bands (given in red)
- Ready-to-use – premixed with 6X Orange Loading Dye for direct loading and room temperature storage
- Supplied with loading dye for sample DNA
|6X Orange DNA Loading Dye||10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol, and 60 mM EDTA.|
|Storage and Loading Buffer||10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF, 10% glycerol.|
|Storage Condition||-20 C|
|Storage Note||Store at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C|
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