ORA™ qPCR Green ROX L Mix

APPLICATIONS

BENEFITS

 qPCR assays based on specific probes: including TaqMan

 Quantification of gDNA, cDNA, viral DNA, low copy number genes, gene expression analysis

 Universal - both standard and fast cycling, all probe qPCR assays, GC or AT rich templates

 Excellent for both single-plex & multiplexing

 Rapid extension, early Ct

PRODUCT DETAILS

highQu qPCR mastermixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates, in multiplexing and guaranty rapid extension with early Ct values with minimum or no optimization.

Our mastermixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the ORA™qPCR Probe Mixes are available in three versions – without ROX, with low or high ROX concentration.

PERFORMANCE

PROTOCOL

 Use special primer selection programs for good planning.

 Work with amplicons in a range of 80-200, max 400 bp.

 Take typical measures to prevent PCR cross over contamination, keep your bench clean, wear gloves, use sterile tubes and filter pipet tips.

 Run reactions in triplets; include a no-template control and positive control in parallel.

 Thaw and keep reagents on ice. Mix well before use.

 Do not perform annealing/extension for more than 30 seconds and do not use lower than 60 °C temperature for this step.

Cycles